Kev Koom Tes Sib Koom Tes nrog cov tub ceev xwm kev lis kev cai
Kev tsim tsa ntawm ntau nucleic acid kuaj tau rau avian tus kab mob siab e
Qhov nyob nram qab: Muaj qhov siab xav rau nucleic acid kuaj los ntawm Tuam Tshoj cov kev lis kev cai nkag thiab tawm cais tubrobe chav tsev.
Vim tias qhov tshwj xeeb ntawm cov txheej txheem kev lis kev cai, tsuas yog cov txheej txheem teev hauv cov qauv kev lag luam hauv tebchaws lossis kev lis kev cai tuaj yeem siv rau kev xeem thiab muab cov txiaj ntsig.
Cov xwm txheej tam sim no:
Tam sim no, lub nucleic acid kuaj txoj kev siv los ntawm cov kev lis kev cai PC yog feem ntau cov pa PC, Nested PCR, thiab fluorescence leestitative pcr.
PV thev naus laus zis ntawm cov cuab yeej loj, thiab cov hnoos qeev yuav tsum xa mus rau cov qauv ua haujlwm rau kev sim, uas yog cumbersome thiab sijhawm siv sijhawm.
Kev yuam:
Mira thev naus laus zis, tagnrho rau cov kev xeem ceev ceev, tau txais kev lees paub los ntawm cov neeg siv kev lis kev cai. Txhawm rau kom tau raws li kev thov rau tsiaj nkag thiab tawm cais tawm, peb sib koom tes los tsim cov khoom ua Mira.
Peb muab kev txhawb nqa kev los pab tsim cov qauv kev lag luam thiab txhawb MAA daim ntawv thov hauv cov kev lis kev cai.
| Amingification Technical |
Kha ci |
RT-QPCR |
Zes pccr |
|
Qhov kub thiab txias |
42 Qib |
50 Qib {-95 degree -95 degree -60 degree |
Firestren-pcramplification: 50 degree {{}} degree {{{{{{{{} {8} degree |
|
Cov Khoom Siv Yuav Tsum Tau Ua |
OototherMal Fluorescence Nrhiav tau cov cuab yeej lossis QPC cov cuab yeej |
QPCRESS |
PCR yuam, Electrophoresis System, Gel Kev Ntsuas Hluav Taws Xob |
|
Lub sijhawm ua kom sov |
20min |
90Min |
200 min |
|
Txheem Tshuab rhiab |
20 daim ntawv / μl |
20 daim ntawv / μl |
{{0 {0}}. 8 daim / μl |
|
Nqi zog-ua hauj lwm |
★★★★★ |
★★★ |
★ |
NO1: 2500copies / μl NOT2: 500copies / μl
NO3: 100copies / μl NO4: 20Copies / μl
No5: 4copies / μl No6: 0. 8copies / μl