Cov lus tshaj tawm cov tshiab microfluidic nti thev naus laus zis rau ntau qhov nrhiav tau ntawm CUPENG ALROORGANGANCE. Cov pab pawg neeg tshawb fawb ua ke piv txwv ntau yog yog qhov tseeb sai nucleic annification (MANA) thiab lub sijhawm fluorescence nrhiav. Qhov nti microfluic no muab cov kev siv nrawm nrawm, nkag siab thiab cov tshuaj zoo rau kev kuaj pom ntawm cov kab loj me me.
Kev Tshawb Nrhiav Cov Keeb Kwm thiab Tseem Ceeb
Lub ntiaj teb cws ua liaj ua teb ua liaj ua teb yog ib qho tseem ceeb tshaj cov nyiaj txiag hauv aquaculture, tab sis kev lag luam tau ntsib kev hem thawj ntawm pathogenic microorganisms. Cov kab ke no feem ntau ua rau kev tuag loj-tuag ntawm cov cw, muaj feem xyuam rau kev tsim khoom thiab nyiaj txiag. Xws li tus kab mob HepatoseCancreatic Necrosetic (WSSTATOPY), thiab lwm yam kab mob Hypodicus (EHP), thiab lwm yam kab mob dawb, thiab lwm yam kab mob
Tsoos Partogenection txoj kev kuaj pom, xws li polymerase cov tshuaj tiv thaiv (PV thev naus laus zis), muaj qhov muaj txiaj ntsig zoo, tab sis yog qhov ntev, thiab cia siab rau cov khoom siv hauv tes. Cov kev txwv no ua rau PCR kev tsis tsim nyog rau kev kuaj mob sai sai. Mira Technology tau dhau los ua cov molecular nrhiav cov cuab yeej haum rau ntawm-qhov chaw thov vim nws cov yam ntxwv sai sai ua rau kub thiab txias.
Mira Technology Share
Ntau tus enzyme constant-kub ceev nucleic acid amomicial thev naus laus zis ntawm ntau qhov kev ua haujlwm kub ntawm chav kub kom ua tiav kev ua kom sai ntawm nucleic acids. Nws yog ib qho tseem ceeb heev ntawm lub chaw ceev nucleic acid tabsis kev siv tshuab. Piv nrog PCR, MMA muaj ntau dua zoo dua.
Tsim thiab kev hloov tshiab ntawm microfluidic chips
Schematic daim duab ntawm RPA nrhiav kom tau platform ntawm chip rau ob peb qhov tseem ceeb Shrimp cov kab mob pathogenic microorganisms
(A) qauv ntawm ib qho disc-hom microfluidic nti. 1: Cov tshuaj tiv thaiv kev tawm tsam; 2: pob valve; 3: pa tawm chaw nres nkoj; 4: Cov chaw nres nkoj uas kuaj kab hluav taws;
(B) pom qhov pom. 5: Cov yeeb yaj kiab tshav kub los yog kub 6: nti substrate.
(C) cov ncauj lus kom ntxaws ntawm rau 6 teev ntawm cov primers hauv lub sector. Txhua qhov txheej txheem txheej txheem yog ua ntej-tsau hauv cov tshuaj tiv thaiv chamber.
(D) cov txheej txheem ua haujlwm tiav ntawm-nti RPA nrhiav lub platform.
Cov lus tshaj tawm cov cim microfluidic sib xyaw nrog MMA thev naus laus zis rau ntau qhov nrhiav tau ntawm CURNP cov kab mob pathogenic microorganisms. Cov thev naus laus zis microfluidic tuaj yeem tswj cov kua hauv micron-theem rank manipulation, yog li ua tiav tau zoo thiab tsawg tus nqi nrhiav tau.
Lub nti muaj cov qauv tseem ceeb hauv qab no:
● Centrifugal microfluidic nti
Cov nti los ua ib qho centrifugal tsav tsim qauv, siv centrifugal quab yuam mus rau tusyees faib cov qauv rau txhua qhov tshuaj tiv thaiv. Tus qauv tsim kom yooj yim txoj haujlwm, thiab tus neeg teb xov tooj tuaj yeem ua tiav tag nrho cov tshuaj tiv thaiv los ntawm kev yooj yim txhaj cov qauv.
● Ntau hom kev kuaj pom
Cov nti tau ua ntej-kos nrog cov kab mob kab mob uas yuav tsum tau kuaj pom: Tus kab mob HSSV), v-aypitirus (shiv) thiab enterohepatitis hepatica (EHP). ) Primer pretres rau tsib pathogens. Cov qauv tsim no tau txuas ntxiv rau cov hom phiaj gene hauv ib qho tshuaj tiv thaiv, ua rau pom kev nrawm nrawm ntawm ntau kab mob.
3D Lub Sijhawm Flumescence Flu
Lub Mira Technology Qhov kev tiv thaiv Amplification hauv lub nti ua ke nrog kev sojntsuam fluorescent, uas tuaj yeem soj ntsuam qhov kev kawm ntawm lub zog ntawm lub sijhawm. Cov kev sojntsuam cov teeb meem tso cai rau lub teeb liab fluorescent kom raug tso tawm tsuas yog thaum cov khoom lag luam zoo nkauj yog tsim tawm, yog li kawm tau nrawm nrawm thiab rhiab tau kom muaj kev pom kom meej.
● Cov ntsiab lus tsawg dua
Txhua cov tshuaj tiv thaiv ntawm cov nti ntawm cov tshuaj tiv thaiv tsuas yog yuav tsum muaj cov tshuaj tiv thaiv ntawm 5 μL, uas tau txo reagent noj. Thaum tag nrho cov txheej txheem tau khiav, nce mus txog plaub qhov piv txwv tuaj yeem ua tiav ib txhij, thiab txhua tus qauv tuaj yeem tshawb pom tsib pathogens, kev txhim kho kom tau txais kev ua tau zoo.
Rhiab, feem ntau thiab rov ua dua
Kev ua yeeb yam ntawm lub kaw lus nti suav nrog kev kuaj pom ntawm cov kev ua si, resonance thiab rov ua dua.
Sensitivity
Kev sim ua kom pom tau tias lub nti tuaj yeem tshawb pom lub hom phiaj tshuaj Qhov kev siab zoo no ua kom paub tseeb tias cov cuab yeej thev naus laus zis tuaj yeem ntes cov tib neeg muaj mob thaum ntxov hauv kev siv cov pathogens thaum ntxov.
Prophemity Tshwj Xeeb
Qhov kev sim ua rau hla kev ntsuas kev tawm tsam ntawm ntau yam kev ua haujlwm tsis muaj txiaj ntsig zoo rau lub hom phiaj pathogens thiab tuaj yeem paub tseeb ntau hom pathogens. Txhua qhov kev sim rau tus kab mob Hepatosoprequic (WSSVCTIVE) Zam kev ua tsis tau zoo.
● Kev Rov Ua Dua
Txhawm rau ntsuas qhov ruaj ntseg ntawm cov qauv, cov pab pawg tshawb fawb tau ua ntau yam kev sim ua. Cov txiaj ntsig tau pom tias cov coefficient ntawm kev sib txawv (CV tus nqi) ntawm cov kev sim sib txawv yog tsawg dua 10%, qhia qhov kev sim ua kom ua tau zoo thiab muaj txiaj ntsig.
Daim teb daim ntawv thov thiab cov txiaj ntsig
Tus tsim ntawm Mira Technology microfluidic nti muab rau nws tseem ceeb daim teb thov kev zoo, tshwj xeeb tshaj yog tsim nyog rau kev nrhiav kom paub sai thiab saib xyuas cov pathogens hauv kev ua liaj ua teb shrimp:
● Cov txheej txheem kev ua haujlwm yooj yim
Cov txheej txheem kev tshawb xyuas tsuas yog yuav tsum tau txhaj cov qauv rau hauv lub nti thiab pib cov khoom siv tshuaj tiv thaiv. Nws yuav tsum tsis txhob muaj kev ua haujlwm tsis muaj kev ua haujlwm thiab haum rau cov kws tshaj lij uas tsis siv rau ntawm qhov chaw.
● Cov lus teb sai sai
Lub kaw lus tuaj yeem tau txais cov lus tau kuaj tsis pub dhau 20 feeb, luv luv ntawm lub sijhawm xeem. Qhov no yog qhov tseem ceeb rau cov kab mob sai sai teb hauv kev ua liaj ua teb thiab txo tus kis ntawm pathogens.
● NTAU LUB CEV
Tsib lub athogens tuaj yeem txheeb xyuas hauv ib qho kev sim, uas txhim kho kev siv tau zoo thiab txuag tau cov nqi thiab sijhawm.
Mira Technology Prospaps
Txawm hais tias PCR thev naus laus zis tau ntev raws li tus qauv kub rau lub peev xwm MMA yog qhov kev ua haujlwm sai ntawm qhov kub thiab txias, thiab cov khoom siv tau yooj yim. Los ntawm kev sib piv nrog pcr ke nrog DNA ua ntu zus, nws tau pom tias cov txiaj ntsig tau ntawm Mira yog qhov tshwj xeeb ntawm cov thev naus laus zis ntawm MMA thev naus laus zis tau ua kom luv. Yog li no, MMA cov chips yog suav tias yog cov thev naus laus zis ntau dua rau cov ntawv thov uas yuav tsum tau nrhiav kom nrawm dua.
Kev siv tshuab hluav taws xob microotic yog cov khoom siv tau zoo tshaj plaws (MANA) cov cuab yeej siv tau zoo (MMA) cov cuab yeej siv tau zoo (MANA) cov cuab yeej siv tau zoo) Cov cuab yeej siv txiaj ntsig zoo rau kev kuaj cov kab mob ntawm cov kab mob cws. Cov thev naus laus zis no muaj qhov zoo ntawm cov kev xav zoo, qhov tshwj xeeb, kev ua haujlwm nrawm, thiab muaj kev tiv thaiv yooj yim hauv aquaculture tiv thaiv thiab tswj. Nrog kev txhim kho ntxiv thiab ua kom zoo dua ntawm thev naus laus zis, lub kaw lus yuav tsum tau siv rau kev kuaj pom ntawm cov tsiaj tiv thaiv tsiaj ntau thiab saib xyuas dav dav.
Cov txiaj ntsig kev tshawb fawb tau muab txoj kev pheej hmoo ua kom muaj kev pheej hmoo ua rau muaj kab mob aquaculture, thiab tso cov kev txhim kho ntawm cov aquaculture.