Kev tshawb xyuas dual ntawm kev hloov kho cov qoob loo siv cov thev naus laus zis thiab CAS12A / Cas13a
- Ntawv:Development of a novel Cas13a/Cas12a-mediated 'one-pot'dual detection assay for genetically modified crops
- Phau Ntawv Journal:Phau ntawv Journal ntawm kev tshawb fawb tshaj plaws
- Cuam tshuam Factor:11.4
- Khoom npe:DNA LSothermal Sai Snorification Cov Khoom Siv (Theem Pib)
- Catalog tsis muaj:Wlb8201kit
| Yam haujlwm | Txojkev | Fluorescent sojntsuam lab ntawv | Kuaj Sawb sojntsuam Tshwm | Sij hawm | Kev hnov tau |
| Nrhiav tau ntawm camv35s thiab nos gene nyob rau hauv kev hloov pauv ntawm cov qoob loo |
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35 min | 20Copies / Kuaj |
Mira sib xyaw nrog crispr
- Kawm ntawm ib {- Tube txoj kev sib txuas ua ke, yog qhov ua kom sov, yog cas12a. Tshooj: Ceev ceev thiab rhiab nrhiav tau ntawm sars- teg-} kev siv suboptimal protostrays nyob ib sab motifs rau cas12a
- Ntawv:Xwm Biomedical Engineering
- Cuam tshuam Factor:29.2
- Khoom npe:DNA LSothermal Sai Snorification Cov Khoom Siv (Theem Pib)
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Yam haujlwm |
Txojkev |
Sij hawm |
Kev hnov tau |
Ua yeeb yam |
Tsab ntawv |
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Coronavirus (covrid -} 19) - orks gene, n gene, thiab gene HPV18 HCMV (tib neeg cytomegalovirus)
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Ib -}} Tshawb nrhiav MIRA yog qhov ua kom sov thiab Cas12A centavage system Tshawb xyuas qhov cuam tshuam ntawm suboptimal Pam thaj tsam ntawm kev sib tw sib tw ntawm nucleic acid amplification thiab cov kev sib tw ntawm ib {}}}} Tshawb nrhiav. Txoj kev no tau siv thoob plaws cov kab sib txawv thiab cov caj ces, thaum kawg tsim kev tswj hwm ntawm txoj kev spamc. |
20min |
1copy / μl |
Rhiab: 94.2% Tshwj Xeeb: 100%
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Piv piv cov kev ua tau zoo ntawm amp yav tom ntej reagents thiab lwm yam reagents. AMP reagents tau zoo dua rau cov txheej txheem cas. |
