Cov khoom siv no sib zog multienzyme yog qhov tseeb sai sai (Mira) thev naus laus zis, kev ua haujlwm ntawm chav tsev kub lossis qis li 42ºC. Los ntawm kev siv cov protein thiab ib leeg-strand khi cov protein, cov ntawv tso cai thiab cov ntawv tshaj tawm uas tshawb nrhiav thiab khi rau lub hom phiaj hom neeg homologous. Cov txheej txheem no ua rau muaj kev tsim ntawm D-Voop cheeb tsam ntawm thaj chaw homologous, ua rau strand kev sib pauv. Raws li cov kev ua haujlwm tsis sib xws los ntawm cov complex, cov kab polymerase mus rau 3 'qhov kawg ntawm tus thawj pib, pib sib tw txuas ntxiv. Mira Technology yog qhov zoo rau kev sim DNA amplification thiab lwm yam kev pab cuam txais tau, muab cov kev daws teeb meem txhim khu kev qha rau cov kev tshawb fawb molecular.
【Nta】
High rhiab heev & muaj zog tshwj xeeb
Ua tiav txhim khu kev qha thiab meej DNA amplification.
Kev ceev ceev lub sijhawm
Ua rau 30 feeb.
Yooj yim siv
Cov tshuaj tiv thaiv yog muab rau hauv cov hmoov hmoov kom qhuav, ua haujlwm yooj yim rau cov haujlwm thiab kev cia khoom.
Tsawg-hassle teeb
Tsis tas yuav siv cov tshuab ntim pcr. Cov tshuaj tiv thaiv tuaj yeem ua tau siv cov hlau uas pheej yig lossis dej da dej, ua kom nws raug nqi rau ib qho lab.
【Qhov tsim thawj tsim qauv】
Rau kev ua tau zoo tshaj plaws, peb pom zoo primers nrog ib tug ntev ntawm 30-35 bp. Cov primers luv luv yuav txo qhov amplification ceev thiab nrhiav tau cov rhiab. Txhawm rau kom ntseeg tau txiaj ntsig zoo, cov ntawv tso cai yuav tsum tsim kom tsis txhob muaj cov qauv txheej txheem theem nrab uas tuaj yeem cuam tshuam nrog tus txheej txheem. Qhov zoo tshaj plaws Ampicon ntev yog nruab nrab ntawm {1}} bp rau cov txiaj ntsig zoo tshaj plaws.
【Cov Specifications】
- Cov Lus Qhia Tshwj Xeeb: 48 T / PAC
【Khoom Siv Hluav Taws Xob】
|
Muaj pes tsawg |
Zoo siab |
|
Tsis |
1.6ml × 1tube |
|
B tsis tsev |
150μl × 1Tube |
|
Kev Tswjhwm Zoo |
100μl × 1tube |
|
Kev tswj hwm qhov zoo tshaj tawm |
60μl × 1Tube |
|
Reagent |
48 T |
|
Kaus Los Ntawm Txhais Tes |
1 daim ntawv theej |
【Khoom muag khoom】
- Kev Cia Cai Cia:Khaws rau ntawm tsawg dua lossis sib npaug rau {{0} ºC nyob rau ib puag ncig kub tas li. Cia kom deb ntawm lub teeb thiab tsis txhob muaj lub siab hnyav.
- Txee Lub Neej:14 lub hlis.
【Tsab ntawv】
- Kev Tsim Nyog Tus Kheej / kev yuav tsum muaj ntxiv rau kev txhim kho Mira txoj haujlwm.
- Cov khoom siv muaj nyob hauv ob hom - DNA thiab RNA. Hom RNA suav nrog transcriptasease, tso cai ua kom sov nrog RNA, DSDDNA, thiab SSDNA ua tus qauv. Cov hom DNA tuaj yeem tsuas yog ua kom pom tseeb nrog DSDNA raws li tus qauv.
- Muaj nyob hauv peb hom - theem pib, thiab kuaj kab txaij. Xaiv cov khoom siv uas tsim nyog raws li koj cov kev xav tau thiab siv cov primers / primes.
Cim npe nrov: Mira DNA yog nthuav tawm cov khoom siv sai sai, Tuam Tshoj MMA DNA yog nthuav tawm cov khoom siv yooj yim, cov chaw muag khoom
