Cov khoom siv no siv Isottermal nucleic acid devicate tshuab uas ua haujlwm nyob rau hauv chav tsev kub thiab muaj qhov sib txawv tas li (feem ntau 39 degree -42 degree). Cov txheej txheem pib nrog transcriptase synthesizing DNA (CDNA) los ntawm cov qauv RNA uas siv cov ntawv primers tshwj xeeb. Cov complex tsim los ntawm kev ua tsis tau zoo thiab cov primers, pab los ntawm cov protein thiab ib leeg-strand khi cov protein, tshawb nrhiav thiab khi rau lub hom phiaj homology sau. Qhov no ua rau muaj kev tsim ntawm D-Voop cheeb tsam ntawm chav tsev homologous, pib strand txauv. Thaum excombinase distociates, polymerase khi rau 3 'kawg ntawm tus thawj pib, pib sib tw txuas ntxiv. Cov khoom siv kuj siv tau exonucquase kev ua ub no thiab kev coj ua molecular rau kev saib xyuas tiag tiag ntawm cov txheej txheem kev ua kom pom cov khoom fluorescence.
【Nta】
Siab rhiab
Tsim los ua kom meej rna rna amplification, kom paub tseeb tias nrhiav tau.
Kev yoov mov
Cov tshuaj tiv thaiv ua tiav hauv tsuas yog 20 feeb, muab kev qhia sai sai.
Lub nkuaj
Cov hom hmoov kom qhuav yooj yim tuav thiab khaws cia.
Kev sib haum
Tau tshaj thaj tsam ntawm fluorescence pcr kev, tas li kub fluorescent amplີ amplີ amplີ ampl, thiab lwm yam fluorescence nrhiav khoom siv.
【Qhov tsim thawj tsim qauv】
Rau kev ua tau zoo tshaj plaws, Primers yuav tsum tau 30-35 BP nyob rau hauv ntev. Cov ntawv luv luv tuaj yeem cuam tshuam kev nrawm nrawm thiab rhiab. Cov primers yog tsim kom tsis txhob muaj cov qauv theem nrab uas yuav cuam tshuam rau txheej txheem ua kom zoo. Cov lus pom zoo kom zoo yog nyob nruab nrab ntawm 150-500 bp.
【Fluorescent sojntsuam tsim】
Qhov kev soj ntsuam ua ntu zus tsis sib tshooj nrog lub vev xaib tshwj xeeb, thf) nyob rau hauv ib nrab ntawm {}}}}}} lt deb ntawm 5 'kawg li lub paub txog qhov chaw rau exonuclease; Pawg Fluorescent yog cim cov khoom siv THF, ib pawg quenching yog daim ntawv lo hauv qab ntawm ob pawg yog {3}} NT; Thf yog txog 15 nt deb ntawm 3 'kawg, thiab 3' kawg yog sau nrog pawg, xws li pawg Amine, ib qho C 3- lub dav hlau.
【Khoom Siv Hluav Taws Xob】
|
Ckev zais |
Cqho tsis ntsuas |
|
Tsis |
1.6 ml × 1tube |
|
B tsis tsev |
150 μl × 1Tube |
|
Reagent |
48T |
|
Kaus Los Ntawm Txhais Tes |
1Copy |
Nco tseg:Xav txog qhov teeb meem ntawm nucleic acid uniddation, RNA series khoom tsis muab cov qauv tswj zoo thiab prever prever.
【Cov Specifications】
- Cov Lus Qhia Tshwj Xeeb: 48 T / Pob
【Knws cia】
- Kev Cia: Khw muag khoom tsawg dua lossis sib npaug rau {{0} ºC nyob rau ib puag ncig kub tas li. Cia kom deb ntawm lub teeb thiab tsis txhob muaj lub siab hnyav.
- Txee lub neej: 14 lub hlis.
【Tsab ntawv】
- Kev Tsim Nyog Tus Kheej / kev yuav tsum muaj ntxiv rau kev txhim kho Mira txoj haujlwm.
- Cov khoom siv muaj nyob hauv ob hom - DNA thiab RNA. Hom RNA suav nrog transcriptasease, tso cai ua kom sov nrog RNA, DSDDNA, thiab SSDNA ua tus qauv. Cov hom DNA tuaj yeem tsuas yog ua kom pom tseeb nrog DSDNA raws li tus qauv.
- Muaj nyob hauv peb hom - theem pib, thiab kuaj kab txaij. Xaiv cov khoom siv uas tsim nyog raws li koj cov kev xav tau thiab siv cov primers / primes.
Cim npe nrov: Mira RNA yog modothermal sai mit fluorescence -Ii, Tuam Tshoj Mira RNA yog nthuav tawm cov khoom siv sai sai
