"Hybrid Daim Ntawv / PDMS microfluidic ntaus ntawv ua ke nrog RNA extraction thiab excombinase amplification rau kev kuaj pom ntawm notovirus hauv cov zaub mov nthuav qhia
Norovirus (Hunov) belongs) rau cov tsev neeg Caliciviridae thiab yog kis tau los ntawm cov zaub mov tsis huv. Nws yog ib qho ua rau ntawm kev mob siab.
Lub koom haum kis tus poj niam ntawm poj niam Davis lub koom haum ntawm McGill University hauv Canada tau muab tshuaj ntsuam xyuas txog kev kawm txog kev kawm. Cov pab pawg neeg tshawb fawb tau tsim tsa cov khoom siv origrofluific thiab siv los ntawm Norovirus 1 (MNV {}) rau kev sim sim. Cov cuab yeej nqus tau RNA siv daim ntawv tawm ib daim ntawv RNA, nthuav dav cov noob txiv plig ua kom zoo nkauj ua kom pom tseeb cov khoom siv hluav taws xob sai sai, thiab ua yeeb yam tom qab kev ntsuas pom ntawm daim ntawv sawb. Txoj kev kuaj pom tag nrho cov txheej txheem thiab cov txiaj ntsig tau pom, nrog kev xeem, ua tiav 100% tshwj xeeb thiab kev ntsuas kev txwv ntawm 200 PFU / ML. Cov cuab yeej no qhia pom tseem ceeb rau cov kab mob foodborne hauv kev ua liaj ua teb, tshwj xeeb tshaj yog cov chaw muaj chaw taws teeb thiab txwv ib puag ncig.
Sim txuj ci
Cellulose ntawv cov ntawv sau cov ntawv MNV -1} rna, uas tau tso tawm mus rau hauv lub mira reagent. RNA yog thawj zaug-transcribed rau hauv DNA (CDNA) thiab tom qab ntawd ua kom nrov rau hauv ob-stranded DNA (DSDNA). Tom qab, kev soj ntsuam tshwj xeeb rau lub hom phiaj ua ntu zus. Tom qab tus Tetranthadrofuran (Thf) yog cleaved los ntawm endonuclease iv, cov seem uas tseem tshuav tau txuas ntxiv los ntawm polymerase los ua kom tiav hologon. Ampicon tau ntes thiab pom tias yog cov xim hloov ntawm T-Line.
(Schematic daim duab ntawm kev sim cov ntsiab cai)
Kev Txhim Kho Kev Txhim Kho
(Schematic daim duab ntawm daim ntawv / PDMS microfluidic ntaus ntawv)
Cov pab pawg neeg tshawb fawb tsim cov ntawv foos / PDMS microfluidic uas sib xyaw ua ke ntau yam, yog suav nrog kev pom kev pom. Cov cuab yeej no sib xyaw ua ke tag nrho cov txheej txheem, los ntawm cov qauv npaj mus rau qhov kawg npaj kom pom ntawm MNV -1.
Lub microfluidic ua tiav qhov kev tshawb nrhiav tsis pub dhau 35 feeb, nrog kev kuaj pom ntawm 200 PFU / ML. Tus nqi ntawm txhua lub microfluidic yog xam kom yog $ 6.76, ua rau nws paub zoo tshaj plaws ntawm txhua txoj kev paub tau siv los ntawm pab pawg tshawb fawb.
Kev sim tseem ceeb
Cov pab pawg tshawb fawb ntxiv cov kev ua tau zoo ntawm cov qauv microfluidic no rau ntes MNV {}} hauv zaub xas lav thiab raspberries. Lawv qhia txog cov neeg nyoos mnv -1 RNA rho tawm mus rau hauv cov kab mob tshiab, tshem tawm cov kab mob sib txawv RNA extraction thiab lim cov kauj ruam. Lub cuab yeej kuaj tau 170 pfu / g hauv zaub xas lav thiab 230 pfu / g hauv raspberries.
(Rhiab heev ntawm MNV -1}} Nrhiav tau hauv zaub xas lav thiab rasbberries uas siv lub microfluidic ntaus ntawv)
Tsoos ib puag ncig kev rau kev ntes cov kab mob hauv cov zaub mov, yuav tsum muaj cov txheej txheem uas tau txais txiaj ntsig zoo, thiab ua kom tiav ntawm cov khoom noj matrices ua. Ib qho ntxiv, cov txheej txheem ib txwm muaj yuav tsum tau sib txawv, loj, thiab cov cuab yeej kim, ua rau lawv tsis haum rau kev siv hauv cov peev txheej.
Cov cuab yeej no tuav cov nqi tseem ceeb rau kev nrhiav kom ceev ceev ntawm Junov thiab lwm hom zaub mov hauv cov khoom noj khoom haus hauv cov khoom noj khoom haus.
Lus Qhia
Phau Ntawv Journal: thov thiab ib puag ncig microbiology
Tshooj cai: Hybrid Daim Ntawv / PDMS microfluidic ntaus ntawv ua ke nrog RNA extraction thiab excombinase amplification rau kev kuaj pom ntawm notovirus hauv cov zaub mov
Cov Factor: 3.9
Pab Pawg Tshawb Xyuas: Tus kab mob kis kab mob, Poj niam Davis lub koom haum, McGill University