【Khoom npe】Mira Group B Streptococcuse fluorescence fluorescence Fluorescence Fluorescence Fluorescence Fluorescence Cov Khoom Siv (Kev Tshawb Fawb Siv nkaus xwb)
【Cov Specifications】48 T / Pob
【Intawm kev siv】
Cov khoom siv no yog tsim nyog rau dav dav kev siv tau ntawm pab pawg b streptococcuse tsis muaj cov vuam.
Pab B Streptococcus (GBS), tseem hu uaStreptococcus agalactiae, yog ib qho kev xaiv sib deev ib zaug hauv kev ua me nyuam sib deev hauv cov poj niam cev xeeb tub. Nws tuaj yeem sib quas ib ntus, ib sab, lossis pheej ua ntu zus ntawm txoj hnyuv plab thiab muaj me me. Nyob rau hauv qee qhov kev mob, kev daws ntshav plab yuav hloov mus rau hauv pathogenicity, uas ua rau tus kab mob GBS los sis cov menyuam mos.
Cov kab mob kis tau dav thoob ntiaj teb, nrog sib txawv ntawm cov nqi ntawm cev xeeb tub ntawm cov teb chaws sib txawv thiab cov cheeb tsam. Kev ntsuas kev ntsuas ntawm 30 Suav ntawm 30000 thiab 2018, encompining tag nrho ntawm 44,716 tus poj niam cev xeeb tub, tshaj tawm GBS Coloury of 11.3% nyob hauv Suav teb.
Txhawm rau tswj hwm kev tiv thaiv ntawm niam cov neeg muaj kev noj qab haus huv ntawm cov neeg muaj kev pom zoo ntawm cov neeg muaj kev pom zoo ntawm cov kws tshaj lij ntawm Suav teb
【Xyuas kom tau lub hauv paus ntsiab lus】
Cov khoom siv no yog ua raws lub peev xwm txheej ci ridificate ua kom sov siab tshaj plaws uas ua haujlwm ntawm qhov kub tsis tu ncua hauv qhov kub thiab txias. Ntawm lub ntsuas kub ruaj khov (feem ntau 39ºC-42ºC), recombinase protein, thiab DNA cov txheej txheem dna, thiab cov tshuaj tiv thaiv DNA ceev, thiab cov tshuaj tiv thaiv DNA ceev xwm txheej kom meej. Raws li cov khoom lag luam ua kom zoo dua qub, ib qho tshwj xeeb tsim molecular kev sojntsuam tso tawm cov cim fluorescent fluorescent fluoricts raws li kev ua ntawm exonuclease. Cov phiajcim no tuaj yeem kuaj pom thaum lub sijhawm thaum lub sijhawm siv fluorescence fluorescence, ua kom cov qauv ntsuas txuas ntxiv ntawm lub hom phiaj tawg ua kom zoo.
【Siv tau cov cuab yeej】
1. Haum rau kev siv biosystems 7500, QuatSttudio 3/7 Flex thiab lwm yam fluorescent ntau cov cuab yeej siv;
2. Siv tau rau peb lub tuam txhab WL -16- ichi, lossis lwm yam detormal fluorcal fluorescence.
【MCheebtsam AIN】
|
Ckev zais |
Cqho tsis ntsuas |
MAIN CUAB YEEJ YEEM |
|
E Buffer |
1. {0 ml * 2 hlab |
Cov tshuaj tiv thaiv tsis |
|
B tsis tsev |
150 μl * 1 raj |
Mg2+thiab li ntawd |
|
PTswj Template(084) |
100 μl * 1 Raj |
Khoom cua plasmid |
|
Tshuaj tivthaiv |
48T |
Enzymes, DNTPS, thiab lwm yam. |
【Chaw ntim khoom】
1. Kev Cia Khoom Kub Tsawg dua lossis sib npaug rau {{1} ºC
2. Kev siv tau lub sijhawm: 14 lub hlis;
Cim npe nrov: Mira Pab Pawg B Streptococcas fluorescence Fluorescence siv xwb, Tuam Tshoj MMA GROUPL B Streptococcus fluoriccescence Siv tsuas yog tsim cov tuam ntxhab, cov chaw muag khoom
