【Khoom npe】 Mira Chlamydia Trachomatis Nucleic Acid Kuaj Sawb Nrhiav Cov Khoom Siv Mus Nrhiav (Kev Tshawb Fawb Siv Tsuas)
【Cov Specifications】48 T / Pob
【Intawm kev siv】Cov khoom siv no yog tsim nyog rau cov kev tsim nyog tau txais ntawm chlamidia trachomatis.
【MCheebtsam AIN】
|
Ckev zais |
Cqho tsis ntsuas |
MAIN CUAB YEEJ YEEM |
|
E Buffer |
1. {0 ml * 2 hlab |
Cov tshuaj tiv thaiv tsis |
|
B tsis tsev |
150 μl * 1Tube |
Mg2+thiab li ntawd |
|
PTswj Template(003) |
100 μl * 1Tube |
Khoom cua plasmid |
|
Tshuaj tivthaiv |
48T |
Enzymes, DNTPS, thiab lwm yam. |
Ntsuam xyuas tus naj npawb ntawm cov qauv nrog kev tswj hwm tsis zoo thiab muaj txiaj ntsig, thiab ntxiv 37.5 μl e buffer rau txhua qhov khov-qhuav reagent.
【Qauv Txheej Txheemg】
1. Nucleic Acid Extraction
Xaiv lub tsim nyog nucleic acid extraction txoj kev thiab reagents mus rho tawm.
2. Ntxiv 10 μl ntawm nucleic acid template rau cov leeg tawm ntawm lub raj, ntxiv 10μl ntawm kev tswj xyuas zoo rau qhov kev tswj tau zoo, thiab ntxiv 10 μl ntawm cov dej qias neeg;
3. Ntxiv 2.5μl B tsis rau txhua cov tshuaj tiv thaiv cov nqaij, thiab npog lub hau (rau ntau qhov kev tiv thaiv, nws pom zoo ntxiv rau sab hauv ntawm lub hau ntawm cov raj tawm tsam);Invert cov tshuaj tiv thaiv raj 8-10}} sij hawm kom sib xyawL, tom qab sib tov, muab cov tshuaj tiv thaiv cov tshuaj rau hauv qab ntawm lub raj thiab pauv mus rau thaj chaw amplification.
【Nucleic Acid Amplification】
1.Qhov reagent tau nthuav dav hauv ib qho da dej ntawm 39 ~ 42 degree tau txuas ntxiv yog tias cov tshuaj tiv thaiv kev pom zoo, tab sis nws tau pom zoo kom txo qis, thiab nws tau muab cov tshuaj tiv thaiv kab mob Thov xaiv ib qho tsim nyog nucleic acid kuaj tawm mus nrhiav kom tau. Peb lub tuam txhab nucleic cov kua qaub acid muaj ib daim hlab, thiab cov kab kuaj tsis muaj ib daim hlab;
2. Kev tswj tau zoo: ob txoj kab kev tswj hwm zoo thiab cov kab kuaj pom muaj ib daim hlab;
3. Qhov kev sim saum toj no ntawm tib lub sijhawm, lwm yam kev sim yuav suav tau tias tsis yog;
4. Cov kev tswj hwm zoo thiab zoo yuav tsum tau teeb tsa rau kev sim.
5. Txawv hom kev sib tw ntawm cov ntaub ntawv kuaj yuav muaj kev ua tau zoo.
【Chaw ntim khoom】
1. Kev Cia Khoom Cia: Cia kub tsawg dua lossis sib npaug ntawm {{1} ºC, khaws cia kom deb ntawm lub teeb thiab zam kev nyuaj;
2. Kev siv tau lub sijhawm: 14 lub hlis;
Cim npe nrov: Mira chlamydia trachomatis nucleic acid kuaj cov khoom siv











