【Khoom npe】Mira Sars-cekh {1}} (}} (}} (Of1ab Gene / Siv Rnasep) Fluorescence Nrhiav Khoom Siv (Kev Tshawb Fawb Siv Tsuas)
【Cov Specifications】48 T / Pob
【Intawm kev siv】Cov khoom siv no yog tsim nyog rau cov kev paub txaus ntawm cov coronavirus tshiab (SARS-CORE {1}}}}}}}), thiab cov hom phiaj nrhiav tau (endogenesis rnasep gene).
【MCheebtsam AIN】
|
Ckev zais |
Cqho tsis ntsuas |
MAIN CUAB YEEJ YEEM |
|
E Buffer |
1. {0 ml * 2 hlab |
Cov tshuaj tiv thaiv tsis |
|
B tsis tsev |
150 μl * 1 raj |
Mg2+thiab li ntawd |
|
PTswj Template(062) |
100 μl * 1 Raj |
Khoom cua plasmid |
|
Tshuaj tivthaiv |
48T |
Enzymes, DNTPS, thiab lwm yam. |
【Kev Cia Cov Neeg Cia Thiab Hnub So】
1. Kev Cia Khoom Cia: Tsawg dua lossis sib npaug rau -20 ºC tas li taws kub ib puag ncig
2. Txee lub neej: 14 hlis;
3. Saib lub ntim sab nraud rau hnub ntau dua.
【Siv tau cov cuab yeej】
1. Haum rau kev siv biosystems 7500, QuatSttudio 3/7 Flex thiab lwm yam fluorescent ntau cov cuab yeej siv;
2. Siv tau rau peb lub tuam txhab lub chaw kuaj fluorcescence.
【Szoo li yuav tsum tau】
1. Sau cov qauv ntsuas raws li cov qauv muaj feem xyuam;
2. Nws raug nquahu kom xeem sai li sai tau tom qab cov qauv sau. Cov qauv kev xeem tuaj yeem khaws ib ntus ntawm 4ºC tsis pub dhau 24 teev; Rau lub sijhawm ntev, nws yuav tsum khaws cia hauv qab {{4} ºC, thiab pheej rov qab khov thiab yaj yuav tsum zam;
3. Yog tias koj siv tus qauv tshuaj tiv thaiv lossis cov tshuaj tiv thaiv sai los kho cov tshuaj uas muaj cov protein uas cuam tshuam, alkali, surfactant, thiab lwm yam, ntawm kev ua haujlwm ntawm reagent.
Cim npe nrov: Mira Sars-C}}}}}}}}}}}}}}}}} {{4 4 4} {4 4 {4 4} {{4 4} {4 4 4} {4 4 4} {4 4 4} {4 4 4} {
