1. Cov khoom yog siv rau nucleic acid kuaj tau ntawm cov tsiaj kab mob pathogens, thiab cov khoom rau cov kab mob zoonotic tsuas yog rau kev tshawb fawb kev tshawb fawb;

2. Cov khoom lag luam yog qhov tsim nyog rau nucleic acid tau txais cov ntaub so ntswg, cov pob zeb mos, ntshav thiab lwm yam kuaj. Cov kev rho tawm tuaj yeem ua kom muaj nucleic kua qaub siv cov qauv hlaws siv lub ntsej muag, lossis siv peb lub tuam txhab Acid

3. Lub caij nyoog nrhiav tau lub sijhawm DNA / RNA cov hom phiaj nrhiav tau nrhiav kom tau ntau tshaj 20 feeb. Cov khoom lag luam RNA twb muaj cov transcriptor rov qab rau ib-kauj ruam nrhiav kev pom;

4. Ib lub hom phiaj fluorescence nrhiav khoom feem ntau yog cov fluorescence raws. Rau cov khoom siv tsis tau, thov saib cov lus qhia rau kev nrhiav kom tau raws. Yog tias muaj cov kev xav tau tshwj xeeb, nws tuaj yeem kho tau;

5. Ib leeg-lub hom phiaj kuaj tau tsim nyog rau nucleic acid kuaj kab mob rau ntes fluorescein (fluc / tshaib plab) / biotin. Rau cov khoom siv tsis tau, thov saib cov lus qhia rau cov pab pawg hloov kho (tuaj yeem siv nrog peb lub tuam txhab Did Nucleic acid Test Strips). Yog tias muaj cov kev xav tau tshwj xeeb, nws tuaj yeem kho tau;

6. Cov khoom lag luam zoo yog nyob rau hauv-situ khov-qhuav hmoov ua kom qhuav-qhuav hauv {} Tsov raj pcres. Yog tias muaj cov kev xav tau tshwj xeeb, nws tuaj yeem kho tau.

【Khoom npe】 Mira Yersinia Methis (Pla Gene) Nucleic Acid Kuaj Sawb Cov Khoom Siv Mus Nrhiav (Kev Tshawb Fawb Siv Tsuas)

【Cov Specifications】48 T / Pob

【Intawm kev siv】Cov khoom siv no yog rau kev tshawb fawb tsuas yog siv tau xwb.Qhov khoom siv tau haum rau cov kev paub zoo ntawm Yersinia Metis (Pla Gene) tsis muaj tus kab ntawv

【Xyuas kom tau lub hauv paus ntsiab lus】

Cov khoom siv no yog ua raws li qhov kub tsis tu ncua yog cov khoom siv hluav taws xob, ib nrab ntawm cov khoom siv CDNA Lub qhov nucleic nrawm acid amplification kev tiv thaiv. Nrog kev pab ntawm Endonuclidenase, hloov kho cov kev sojntsuam molecular tau nthuav dav nrog tus qauv hloov kho kom tau los ua ob-xaus hloov cov roj acid tawg. Nrog rau kev tsub zuj zuj cov khoom lag luam, nucleic acid kuaj sim tuaj yeem siv rau kev txhais cov txiaj ntsig xim kawg.

【MCheebtsam AIN】

Ckev zais	Cqho tsis ntsuas	MAIN CUAB YEEJ YEEM
E Buffer	1. {0 ml * 2 hlab	Cov tshuaj tiv thaiv tsis
B tsis tsev	150 μl * 1Tube	Mg²⁺thiab li ntawd

Tus naj npawb ntawm cov qauv yuav tsum tau kuaj, tsis zoo thiab muaj kev tswj hwm zoo, thiab ntxiv 37.5 μl e tsis mus rau txhua tus khov-qhuav reagent.

Qauv ua(Cheeb Tsam Zauv)

Nucleic Acid Extraction

Xaiv lub tsim nyog nucleic acid extraction txoj kev thiab reagents mus rho tawm.

Ntxiv 10 μl ntawm nucleic acid template rau cov tshuaj tiv thaiv raj (tus nqi ntawm cov khoom siv tsis huv ntxiv rau kev tswj kom tsis huv, thiab ntxiv 10 μ ntawm dej huv si;

Ntxiv 2.5μL B tsis mus rau txhua cov tshuaj tiv thaiv kev ua rog, thiab npog lub hau (rau ntau qhov kev tiv thaiv, nws pom zoo ntxiv rau sab hauv ntawm lub hau ntawm cov raj tawm tsam);Invert cov tshuaj tiv thaiv raj 8-10}} sij hawm kom sib xyawL, tom qab tov, muab cov tshuaj tiv thaiv tov yog alrished (lossis sai sai centrifuged) mus rau hauv qab ntawm lub raj thiab pauv mus rau thaj chaw amplification.

Nucleic Acid Amplification (Amplification Thaj Chaw)

Qhov reagent tau nthuav dav hauv ib qho da dej ntawm 39 ~ 42 degree rau {2} Lub sijhawm tshuaj tiv thaiv tau qis dua, tab sis nws pom zoo kom tsis pub tshaj 20 min).

Rtus piv txwv li cas

1. 5 feeb.(Cov kev sojntsuam hauv cov khoom siv no yog kev hloov kho, thiab cov dej ntws los ntawm cov txhab acid tsim nyog. Peb lub tuam txhab nucleic acid kuajKab muaj ib daim strips, thiab cov kab kuaj tsis muaj ib daim hlab;

2. Kev tswj tau zoo: ob txoj kab kev tswj hwm zoo thiab cov kab kuaj pom muaj ib daim hlab;

3. Qhov kev sim saum toj no ntawm tib lub sijhawm, lwm yam kev sim yuav suav tau tias tsis yog;

4. Cov kev tswj hwm zoo thiab zoo yuav tsum tau teeb tsa rau kev sim.

5. Txawv hom kev sib tw ntawm cov ntaub ntawv kuaj yuav muaj kev ua tau zoo.

【Cov kev ceev faj】

1. Thov nyeem cov lus qhia kom zoo zoo ua ntej siv thiab nruj me ntsis ua raws li cov kauj ruam ua haujlwm;

2. Qauv kev tuav yuav tsum tau ua kom nruj raws li biosafety specifications. Thaum lub sij hawm sim, cov khaub ncaws ua haujlwm thiab cov hnab looj tes ntawm hnab looj tes yuav tsum hnav, thiab kev sim yuav tsum tau ua ntau qhov chaw kom tsis txhob muaj kev sib kis(Nucleic acid kuaj yuav tsum tau qhib rau kev tsim kho xim, thiab cov pa phem yuav tsum tau nruj me ntsis tiv thaiv);

3. Siv lub reagent nyob rau hauv lub sij hawm siv tau, thiab cov khoom siv yuav tsis muab tov nrog

PTswj Template(003)	100 μl * 1Tube	Khoom cua plasmid
Tshuaj tivthaiv	48T	Enzymes, DNTPS, thiab lwm yam.

【Kev Cia Cov Neeg Cia Thiab Hnub So】

1. Kev Cia Khoom Cia: Cia kub tsawg dua lossis sib npaug rau -20 ºC tas li kub ib puag ncig, kom deb ntawm lub teeb thiab tsis txhob muaj lub zog hnyav;

2. Cov Khoom Siv Hluav Taws Xob: 14 lub hlis;

3. Saib lub ntim sab nraud rau hnub ntau dua.