Sars-cov -2, tus kabmob tus kab mob rau cov teev cov lus qhia txog kev pab pawg -19, tseem tso kev cov nyom tseem ceeb hauv kev tshawb fawb thiab kev tshawb fawb. Kuaj pom tusOrf1ab Genentawm tus kab mob no ua kom paub tseeb meej, thaum lubRnase C NoobUa hauj lwm ua kev tswj hwm sab hauv kom pom cov qauv zoo thiab sim kev ntseeg tau. Sai sai thiab meej meej nrhiav tau ntawm sars-c -2 yog qhov tseem ceeb rau kev tshawb fawb, kev tshawb nrhiav kev tshawb nrhiav kev kuaj mob.
【Npaj】
TusMira Sars-cekh {1}}}}}}} urf1ab gene / siv RNase P fluorescence nrhiav khoom sivyog tsim los rau cov kev paub zoo tau txais ntawm sars-cov {1}} rna. SivMira (Multienzyme yog nthuav tawm sai sai detification) thev naus laus zis, qhov kev pab no ua haujlwmFast, rhiab, thiab txhim khu kev ntseeg nucleic acid amplificationntawm qhov kub tsis tu ncua (39-42 DEGREE), tsis tas yuav ua rau complex thermal cycling. Qhov kev suav nrogRnase C NoobRaws li kev tswj hwm sab hauv kom ua tau zoo ntawm kev sau cov qauv thiab kev ua tiav. Cov khoom siv no tau npaj tsegrau kev tshawb fawb siv nkaus xwbthiab yog qhov zoo tagnrho rau cov ntawv thov hauvKev tshawb fawb txog kev paub txog kev paub veriS, Epidemiological tshawb nrhiav, thiab nucleic acid kuaj tau technology kev txhim kho.
【Daim Ntawv Thov Thaj Av】
- Kev Tshawb Fawb Txog Kev Tshawb Fawb- Kev tshawb fawb ntawm sars-c -2 cov khoom siv caj ces thiab kev sib hloov taug qab.
- Epidemiological Kev Ncig Saib- Saib xyuas kis tau thiab kis tus qauv.
- Kev Loj Hlob Ntawm Nucleic Acid Yuav Tsum Tau Kev Siv Tshuab- Kev tshawb fawb txog kev kuaj mob sai thiab ua tau pov tseg.
TusMira Sars-cekh {1}}}}}}} urf1ab gene / siv RNase P fluorescence nrhiav khoom sivmuajceev ceev, txhim khu kev qha, thiab cov tshuaj rhiab ntauRau SARS-CE {1}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}} ecting kev tshawb fawb adjectionics thiab kis cov kab mob pathogenics.
【Lus Cim】
1. Cov khoom lag luam tsuas yog rau kev tshawb fawb thiab siv rau nucleic acid kuaj tau ntawm cov pa tawm ntawm cov pa kev ua pa;
2. Cov khoom lag luam yog qhov tsim nyog rau nucleic acid tau txais cov qauv no xws li caj pas thiab cov so ntswg. Cov kev rho tawm tuaj yeem ua rau muaj nucleic kua qaub siv cov qauv hauv nruab nrab, lossis siv peb lub tuam txhab ua yeeb yam zoo nkauj;
3. Lub caij nyoog nrhiav tau lub sijhawm DNA / RNA cov hom phiaj nrhiav tau nrhiav kom tau ntau tshaj 20 feeb. Cov khoom lag luam RNA twb muaj cov transcriptor rov qab rau ib-kauj ruam nrhiav kev pom;
4. Ib lub hom phiaj fluorescence nrhiav khoom feem ntau yog cov fluorescence raws. Raws li cov khoom lag luam nrhiav tau pom, thov saib cov lus qhia. Yog tias muaj cov kev xav tau tshwj xeeb, lawv tuaj yeem kho tau;
5. Ib leeg-phiaj xwm kev sim ntaus hom kev kuaj pom cov khoom lag luam yog qhov tsim nyog rau nucleic acid kuaj cov fluorescein (Fluc / tshaib plab) / biotin (biotin). Yog tias muaj cov kev xav tau tshwj xeeb, lawv tuaj yeem kho tau;
6. Cov khoom lag luam zoo yog nyob rau hauv-situ khov-qhuav hmoov khov-qhuav nyob rau hauv ib qho {daim pcr raj. Yog tias muaj cov kev xav tau tshwj xeeb, nws tuaj yeem kho tau.
Cim npe nrov: Mira Sars-CE {1}} n gene / siv rnesase P nucleic acid kuaj cov khoom siv, cov neeg muag khoom
